A quick and easy high performance liquid chromatography method for evaluation of cefixime in human plasma

Introduction Cefixime (Fig. 1) [(6R, 7R, E)-7-(2-(2amino thiazol-4-yl ( 2carboxy methoxy imino) acetamido)-8-oxo-3-vinyl-5thia-1-azabicyclo [4.2.0] oct-2-ene-2carboxylic acid] is belong to third generation of cephalsporin. The cefixime exists in off white crystals, melts over 220-250 ° C and soluble in alcohol (1). An orally active cefixime has outstanding activity against pathogens such as, Anaerobes, Entero bacteria ceae, gram negative species such as Escherichia coli, Klebsiella, Haemophilus influenzae, Branhamella Catarrhalis, Neisseria gonorrhoeae, Serratiamarcescens, Providencia, Haemophilus, and Meningococcus including b-lactamase producing strains (1-3). Along with its broad spectrum antimicrobial activity and stability, cefixime was used as most suitable in proper dosage for adults as well as pediatrics and widely arranged among cephalosporin family in Iran. in spite of its poor lipophilicity and ionization at physiological pH, cefixime is significantly absorbed unaffected after oral administration (4). The absolute oral bioavailability of cefixime is in the range of 22-54%. Absorption is not appreciably customized by the presence of food. Cefixime may therefore be Abstract Cefixime is essential member of orally energetic third generation cephalosporin and has tremendous activity aligned with many pathogens. The virtual bioavailability of a newly industrial dispersible tablet as compared with a recognized identified formulation. A simple and available reversed-phase HPLC method with UV detection has been urbanized and validate for cefixime evaluate in human plasma using a C18 analytical column and a mobile phase of tetrabutylammonium hydroxide (pH 6.5)-acetonitrile (3:1 v/v). The detection wavelength was 280 nm. To method observed major linear response-concentration association all through the cefixime concentration range of 15100 ng/ml, with the average accuracy within-run and between-run values of 97.29% and 99.27%. The average drug recovery from plasma was 98.2% throughout the linear concentration range. The limits of detection (LOD) and quantitation (LOQ) of the method were 5 and 15 ng/ml, respectively. The method is quick, easy, very steady and precise for the partition, assignment, evaluation of cefixime in human plasma.